Sara K. Biladeau
- Courses1
- Reviews1
- School: University of Louisville
- Campus:
- Department: Chemistry
- Email address: Join to see
- Phone: Join to see
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Location:
2301 S 3rd St
Louisville, KY - 40292 - Dates at University of Louisville: May 2017 - May 2017
- Office Hours: Join to see
Biography
University of Louisville - Chemistry
Experience
University of Louisville School of Medicine
Biochemistry and Genetics Course Representative
Sara worked at University of Louisville School of Medicine as a Biochemistry and Genetics Course Representative
University of Louisville School of Medicine
Tutor
Sara worked at University of Louisville School of Medicine as a Tutor
University of Louisville
Graduate Teaching Assistant
Give pre-lab lecture to prepare students for upcoming lab, assist students while performing lab if necessary, grade quizzes and lab reports weekly, grade final at end of semester
University of Louisville
Biochemistry Course Representative
Sara worked at University of Louisville as a Biochemistry Course Representative
University of Louisville
Senior Instructor
Oversee undergraduate and graduate teaching assistants for organic labs (Chem 343 and Chem 344)
University of Louisville Housing
Resident Assistant
Sara worked at University of Louisville Housing as a Resident Assistant
Education
University of Louisville School of Medicine
Doctor of Medicine - MD
University of Louisville School of Medicine
Biochemistry and Genetics Course Representative
University of Louisville School of Medicine
Tutor
University of Louisville
Master of Science (M.S.)
Chemistry
University of Louisville
Doctor of Philosophy (PhD)
Chemistry
Dissertation: Magnetic Field-Induced Intramolecular Cyclization as a Trigger for Nanoparticle-Based Delivery SystemsUniversity of Louisville
Graduate Teaching Assistant
Give pre-lab lecture to prepare students for upcoming lab, assist students while performing lab if necessary, grade quizzes and lab reports weekly, grade final at end of semesterUniversity of Louisville
Biochemistry Course Representative
University of Louisville
Senior Instructor
Oversee undergraduate and graduate teaching assistants for organic labs (Chem 343 and Chem 344)
Publications
Isotopically Coded N-Methoxy Amide Reagents for GC-MS Profiling of Carbonyl Compounds via Mass Spectral Tag Generation
Analytical Methods
Synthesis and application of isotopically labeled N-methoxy-N-(2-aminooxyethyl)-propionate (MAP), a chemoselective carbonyl derivatization reagent, is reported. To exploit the ready measurement of fragments serving as reporter ions in the m/z 32–34 range, MAP is designed to undergo electron ionization (EI)-induced fragmentation to expel labeled ethyl carbenium ions for relative quantifications in multiplexed analyses. A study of the EI-MS fragmentation behavior of a panel of MAP–carbonyl adducts revealed that the N-methoxy amide motif of MAP is highly predisposed to undergo carbonyl alpha cleavage to produce corresponding labeled carbenium ions in the targeted m/z range. Use of the N-methoxy amide functionality decreased undesired (e.g., uninformative) mass spectral fragmentations as well as provided good resistance to cleavage by amines or base relative to ester functionality. These properties should facilitate the use of MAP in multiplexed GC-MS analyses of complex mixtures containing aldehyde and ketone analytes. A representative multiplexed experiment using MAP isotopologues illustrates this approach for quantification of a carbonyl analyte in pooled sample mixtures.
