Resume

• 40123095

  IRB - Biomedical Research (Group 1)

  CITI Program

  A Division of BRANY

• 2019

  Bioengineering and Biomedical Engineering

  Ira A. Fulton Schools of Engineering at Arizona State University

  Biosignals and Processing

  Biochemistry

  Bioethics and the Law

  Microfluidics & Microfabrication

  Intro to Cellular & Molecular Biology

  Bioethics and the Law

  Engineering Communications

  Anatomy & Physiology for Engineers

  General Chemistry

  Biomaterials

  Differential Equations

  Biodevice Engineering

  Applied Programming in MATLAB

  Calculus & Analytic Geometry

  Intro to Biomechanics

  Experimental Methods in Bioengineering

  Graphical Communication in Design

  Investigative Cellular & Molecular Biology Lab

  Emergency Medical Technology

  Electrical Circuits

• 2017.

  Low cost paper sensor for the detection of E. coli in donated human breast milk

  I am working on my Engineering Senior Design Capstone Project with two other women bioengineering students to develop a functional

  robust paper sensor to detect E. coli in breast milk to ensure safe distribution in rural breast milk banks that lack proper handling

  storage

  and pasteurization practices. \n\nWe are being advised by Mother's Milk Bank of San Jose and PATH

  a global health NGO

  to obtain human breast milk for testing and to familiarize ourselves with a better understanding of the breast milk banking process and industry

  and how to best address our sensor to the needs of the bank

  mother

  and community. \n\nWe received full funding from Santa Clara University Roelandt's Grant and full travel grant funding from the Xilianx Grant and Provost Office Grant for exceptional project and social justice benefits to the community.\n\nWe applied to the Global Health and Innovation Conference at Yale University and were selected to present in the Social Impact Lab in April 2017.\n\nWe were one of three engineering groups asked to present our project to the Santa Clara University School of Engineering Advisory Board on May 11

  and received tremendous feedback. \n\nWe also presented our project in the Senior Design Conference on May 11

  2017 and won first place the Bioengineer 1 session. \n\nWe additionally received the Frugal Innovation Hub Humanitarian Award for the most impactful project at the Santa Clara University Engineering Awards Ceremony on June 16

• 2016

  Summer research project in the Center for Health and Military Performance

  O'Connor

  Samantha

  Translational Genomics Research Institute (TGen)

  Global Brigades

  Inc.

  NCAA Grant Alliance Concussion Study

  Walter Reed National Military Medical Center

  Department of Defense Wounded Warrior Games

  Department of Military and Emergency Medicine

  Santa Clara University

  Atreca

  Inc.

  Bethesda

  MD

  •\tShadowed Dr. Paul Pasquina

  Chief of the Integrated Department of Orthopedics and Rehabilitation & Medical Director of the Amputee Program at Walter Reed Army Medical Center. \n•\tAssisted in the production and fitting of leg prostheses in the Prosthesis Fabrication Room.\n•\tObserved surgeries with Dr. David Welling. Scrubbed in on coronary artery bypass surgery and robotic-assisted da Vinci prostatectomy.

  Medical Shadow

  Walter Reed National Military Medical Center

  Uniformed Services University for the Health Sciences

  •\tCompleted research project under LCDR Heath Gasier in the Consortium for Health and Military Performance (CHAMP): Application of Low Dose Inhaled Carbon Monoxide to Attenuate Diet-Induced Obesity and Insulin Resistance.\n•\tUsed fluorescence microscopy to study mitochondrial organization

  mitochondrial membrane potential

  and mitochondrial reactive oxygen species.\n•\tPresented results of research to CHAMP faculty and staff.\n•\tParticipated in rat tissue collection. Performed heart puncture in rats and collected heart

  liver

  kidney

  spleen

  small intestine to send to other labs. Harvested skeletal muscle (soleus and extensor digitorum longus) and epididymal fat pads following Heath Gasier’s protocol to analyze mitochondria morphology

  function and biogenesis and adipocyte morphology between five rat groups.\n•\tLearned and used a sandwich ELISA protocol with protein samples from CHAMP projects.\n•\tPerformed cell culture with C2C12

  MF1 and MF2 cells to observe differences in differentiation when exposed to heat.

  Summer Intern

  Department of Military and Emergency Medicine

  South San Francisco

  California

  •\tOversaw the growth and inventory of tumor cell panels needed for primary and secondary screens. \n•\tRan antibody

  protein western blots for QC.\n•\tPerformed cell-based binding assays to assess anti-tumor efficacy of antibodies.\n•\tOptimized flow cytometry analysis.\n•\tLearned to operate complex instrumentation

  such as the BIOMEK liquid handler. \n•\tAnalyzed and organized data in Prism. \n•\tPresented projects and data in small and large group meetings.

  Research Associate

  Atreca

  Inc.

  Ghana

  Africa

  •\tTraveled to the Central Region of Ghana with pre-med students for three-week medical mission trip.\n•\tConducted public health research in three communities. \n•\tParticipated in medical clinic by taking patient’s vitals and shadowing doctors. \n•\tInterviewed residents in several communities

  focusing on their knowledge of female reproductive health. Data contributed to abstract that was later presented at the University of Arizona by Deanna Joop

  Assistant Clinical Professor at Northern Arizona University. \n•\tBuilt latrines for three families in three different communities.

  Public Health Intern

  Global Brigades

  Inc.

  •\tParticipated in full time summer internship program in biomedical research under Dr. Kendall Van Keuren-Jensen.\n•\tAssisted in isolating RNA from biofluid samples for 3-year Riddell Concussion Project.\n•\tResearched and prepared poster presentation for capstone research symposium on Hemolysis in Aneurysmal Subarachnoid Hemorrhage Cerebrospinal Fluid in the 2015 Helios Scholars Intern Symposium. \n•\tLearned and used several protocols and kits including QuantiChrom Hemoglobin Assay

  Agilent D1000 Screentape

  TruSeq small RNA sample prep and Norgen Urine Exosome RNA Isolation. \n•\tLearned basic bioinformatic skills under mentorship of post-doc Ashish Yeri. \n•\tAttended research presentations by TGen staff and visiting speakers

  and participated in scientific writing projects.

  Helios Scholar

  Phoenix

  Arizona Area

  Translational Genomics Research Institute (TGen)

  United States Military Academy (USMA)

  West Point

  New York

  •\tProctored baseline ImPACT exam (Immediate Post-Concussion Assessment and Cognitive Testing) for new student athletes.\n•\tOrganized and processed blood collection samples for NCAA Concussion team.

  Research Assistant

  NCAA Grant Alliance Concussion Study

  Santa Clara University

  •\tAssisted Dr. Magda Metwally with Differential Equations

  AMTH 106\n•\tGraded student quizzes

  Teaching Assistant

  United States Military Academy (USMA)

  West Point

  New York

  •\tAssisted sports medicine staff with athletes who needed medical treatment during the games. \n•\tObserved wounded warrior athletes compete in wheelchair basketball

  swimming

  sitting volleyball

  and other activities.

  Medical Volunteer

  Department of Defense Wounded Warrior Games

• 2014

  •\tNurse assistant at medical clinic for patients without health insurance.\n•\tTook patient’s vitals for attending physician. \n•\tPracticed my Spanish when patients did not speak English. \n\n

  The Neighborhood Christian Clinic

  Microscopy

  GraphPad Prism

  Microfluidics

  Research

  Microsoft Excel

  Matlab

  Molecular Biology

  Data Analysis

  Western Blotting

  Science

  Microsoft PowerPoint

  Cell Culture

  ELISA

  Polymerase Chain Reaction (PCR)

  Hemolysis in aneurysmal subarachnoid cerebrospinal fluid

  Vasospasm that occurs as a secondary injury due to aneurysmal subarachnoid hemorrhage (aSAH) contributes to delayed morbidity and mortality. The search for a biomarker to detect secondary injury

  such as vasospasm

  has been an ongoing area of investigation. Hemolysed blood from the hemorrhage into cerebrospinal fluid (CSF) samples could obscure results when trying to identify a marker for secondary injury. It is necessary to rule out the potential for the results to be artificially driven by blood contamination. In this project

  hemolysis was assessed by measuring hemoglobin concentration in CSF. We used the QuantiChrom Hemoglobin Assay Kit. The data was first analyzed across patient information

  where t-tests confirmed blood was not a confounding factor. In a secondary study

  we tested how the blood contamination altered the information in CSF by observing the aligned counts to the previously sequenced information for genes uniquely expressed in the bone marrow

  heart and brain across the different samples

  where a general trend of increased expression in increasing hemoglobin concentration was noted. To determine to what extent the RNA expression profiles of CSF differed between samples of different hemoglobin concentration

  we correlated the normalized read counts (of the significant genes from the linear regression graphs) to the low-concentrated 25th percentile of total samples and the high-concentrated 75th percentile of total samples. Therefore

  the genes identified as significantly differentially expressed relative to hemolysis would not serve as good markers of injury

  and should be carefully considered. These data show that there are transcripts that we can use to measure the blood contamination in our samples

  and that this contamination does not correlate with our markers of vasospasm.

• 2013

  Bachelor of Science (B.S.)

  Bioengineering

  Santa Clara University

• 2011

  •\tAssisted in hospital library gathering information from journals for doctors.\n•\tAssisted nurses in Labor and Delivery Unit and interacted with patients as necessary. \n

  Scottsdale Healthcare is now HonorHealth

• 2009

  High School Diploma

  Varsity Soccer

  Xavier College Preparatory