Nicole King

 NicoleM. King

Nicole M. King

  • Courses3
  • Reviews5

Biography

Macomb Community College ALL - Biology


Resume

  • 2008

    Ph.D.

    The Role of Oxidative Stress in the Pathophysiology of Ovarian Cancer\n\n

    Physiology with a concentration in Reproductive Science

    Wayne State University School of Medicine

    Responsible Conduct of Research (RCR)

    Wayne State University Graduate School

  • 2001

    American Association for Cancer Research

    National Postdoctoral Association

    American Society for Reproductive Medicine

    Michigan Physiological Society

    Bachelors of Science

    Biology

    Wayne State University

  • Home

    Saed's Laboratory at WSU

    Saed Laboratory

    Document Preview

    This is a short preview of the document. Your library or institution may give you access to the complete full text for this document in ProQuest. Once connected

    you can view documents in full as well as cite

    email or print them. You can also find...

    Document Preview

    RNA isolation

    Immunohistochemistry

    Tissue Culture

    SDS-PAGE

    Confocal Microscopy

    Biochemistry

    Cell Culture

    Immunoprecipitation

    Subcloning

    Cell Biology

    Molecular Biology

    Fluorescence Microscopy

    Protein Chemistry

    Primer Design

    RT-PCR

    Transfection

    Northern Blotting

    Western Blotting

    cDNA

    qPCR

    Oxidative stress: a key regulator of leiomyoma cell survival.

    Oxidative stress: a key regulator of leiomyoma cell survival.

    Anti-Müllerian Hormone (AMH) May Stall Ovarian Cortex Function Through Modulation of Hormone Receptors Other Than the AMH Receptor.

    Sox2 gene amplification significantly impacts Overall Survival (OS) in Serous Epithelial Ovarian Cancer

    Updates of the role of oxidative stress in the pathogenesis of ovarian cancer.

    The Role of Angiogenesis in the Persistence of Chemoresistance in Epithelial Ovarian Cancer.

    Molecular Basis Supporting the Association of Talcum Powder Use With Increased Risk of Ovarian Cancer

    Shifting anaerobic to aerobic metabolism stimulates apoptosis through modulation of redox balance: potential intervention in the pathogenesis of postoperative adhesions.

    Advances in the pathogenesis of adhesion development: The role of oxidative stress

    Chapter: New Insights into the Pathogenesis of Ovarian Cancer: Oxidative Stress

    Nicotinamide adenine dinucleotide phosphate oxidase is differentially regulated in normal myometrium versus leiomyoma

    Xenotransplantation of pre-pubertal ovarian cortex and prevention of follicle depletion with anti-Müllerian hormone (AMH)

    Adhesion phenotype manifests an altered metabolic profile favoring glycolysis.

    Specific point mutations in key redox enzymes are associated with chemoresistance in epithelial ovarian cancer.

    A Single Nucleotide Polymorphism in Catalase Is Strongly Associated with Ovarian Cancer Survival.

    OBJECTIVE:\nThe objective of this study was to determine the expression

    and effect of targeting CD11b with a monoclonal antibody in ovarian cancer cells.\n\nMETHODS:\nCD11b expression was determined in epithelial ovarian cancer (EOC) cell lines and tissues by immunofluorescence and flow cytometry. Cytotoxicity of the CD11b antibody and synergism with chemothearapeutic drugs were determined by the MTT Cell Proliferation Assay in human macrophages

    normal ovarian epithelial cells

    and in both sensitive and chemoresistant EOC cell lines. Cell migration was assessed with a scratch assay and in vivo effects of the CD11b antibody was assessed with a nude mouse ovarian cancer xenograft model. Data was analyzed with either t-tests or one-way ANOVA.\n\nRESULTS:\nCD11b was unexpectedly expressed in several EOC lines and tissues

    but not normal tissues. Targeting CD11b with its monoclonal antibody resulted in intriguing cytotoxic effects in sensitive and chemoresistant EOC lines

    while surprisingly not affecting normal cells. More importantly

    the cytotoxicity of the CD11b antibody when combined with chemotherapeutic drugs (cisplatin or docetaxel) was significantly synergistic

    in both sensitive and chemoresistant EOC cells. The anti-tumorigenic effect of the CD11b antibody was confirmed in an ovarian cancer nude mouse xenograft model.\n\nCONCLUSION:\nHere we identify CD11b as a novel target

    which selectively induces cytotoxicity in ovarian cancer cells.

    Novel expression of CD11b in epithelial ovarian cancer: Potential therapeutic target.

    Evitar (l-Alanyl-l-Glutamine) Regulates Key Signaling Molecules in the Pathogenesis of Postoperative Tissue Fibrosis

    Dr. Ghassan Saeddetti

    Goserelin fosters bone elongation

    but does not prevent ovarian damage

    in cyclophosphamide-treated pre-pubertal mice

    Anti-Mullerian hormone (AMH) regulates stemness-promoting factors in fresh and previously vitrified-warmed ovarian cortex

    The Creation of a Model for Ex Vivo Development of Postoperative Adhesions.

    Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development

    King

    Macomb Community College

    Wayne State University School of Medicine

    DS Biotech

    LLC

    Medpace

    Detroit

    - Laboratory Management.\n- Investigating the role of oxidative stress in the pathogenesis of benign gynecologic disorders

    including postoperative adhesions and fibroids as compared to ovarian cancer. \n- Determining the molecular mechanisms underlying the acquisition of chemoresistance in ovarian cancer.

    Postdoctoral Fellow

    Wayne State University School of Medicine

    Cincinnati

    Ohio

    Clinical Research Associate

    Medpace

    Center Campus

    Current courses taught:\nBIOL1000: An introductory lecture and laboratory course in basic biological principles aimed at an understanding of the life processes common to all living things. The major areas of emphasis include the chemical and cellular basis of life

    reproduction

    growth

    development

    heredity

    evolution

    and ecology.

    Adjunct Faculty

    Macomb Community College

    Greater Detroit Area

    Research Assistant

    Wayne State University School of Medicine

    Detroit

    Laboratory management.\nConduct research using state of the art molecular and cellular biology

    and biochemistry techniques to investigate the role of oxidative stress in the pathogenesis of ovarian cancer.

    Graduate Research Assistant

    Wayne State University School of Medicine

    Detroit

    - Laboratory Director\n- Evaluation of markers of oxidative stress

    inflammation

    metabolism

    cell proliferation and apoptosis in the pathogenesis of various diseases\n- Project Management\n- Budgeting

    Director of Laboratory Operations

    DS Biotech

    LLC