Kelsey L. Poulson-Ellestad
- Courses2
- Reviews3
- School: Roosevelt University
- Campus:
- Department: Chemistry
- Email address: Join to see
- Phone: Join to see
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Location:
430 S Michigan Ave
Chicago, IL - 60605 - Dates at Roosevelt University: January 2016 - April 2018
- Office Hours: Join to see
N/A
Would take again: Yes
For Credit: Yes
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Awesome
Dr. Poulson is an awesome professor. She is very friendly and approachable. The course is not easy, but Dr. Poulson makes sure to clarify any confusion there is in the class and she takes her time explaining things. She genuinely wants her students to succeed and will help you in any way possible. I definitely recommend taking her class!
Biography
Roosevelt University - Chemistry
Experience
Georgia Institute of Technology
Graduate researcher
Kelsey worked at Georgia Institute of Technology as a Graduate researcher
Woods Hole Oceanographic Institution
Postdoctoral Investigator
Kelsey worked at Woods Hole Oceanographic Institution as a Postdoctoral Investigator
Roosevelt University
Assistant Professor
Kelsey worked at Roosevelt University as a Assistant Professor
University of Wisconsin Milwaukee
Research Technician
Kelsey worked at University of Wisconsin Milwaukee as a Research Technician
Education
Georgia Institute of Technology
Doctor of Philosophy (Ph.D.)
Biology, General
Georgia Institute of Technology
Graduate researcher
University of Wisconsin-Milwaukee
Bachelor of Science (BS)
Biology & Biochemistry
Publications
enhancement of antibiotic activity against multidrug-resistant bacteria by the efflux pump inhibitor 3,4-dibromopyrrole-2,5-dione isolated from a pseudoalteromonas sp.
journal of natural products
members of the resistance nodulation cell division (rnd) of efflux pumps play essential roles in multidrug resistance (mdr) in gram-negative bacteria. here, we describe the search for new small molecules from marine microbial extracts to block efflux and thus restore antibiotic susceptibility in mdr bacterial strains. we report the isolation of 3,4-dibromopyrrole-2,5-dione (1), an inhibitor of rnd transporters, from enterobacteriaceae and pseudomonas aeruginosa, from the marine bacterium pseudoalteromonas piscicida. 3,4-dibromopyrrole-2,5-dione decreased the minimum inhibitory concentrations (mics) of two fluoroquinolones, an aminoglycoside, a macrolide, a beta-lactam, tetracycline, and chloramphenicol between 2- and 16-fold in strains overexpressing three archetype rnd transporters (acrab-tolc, mexab-oprm, and mexxy-oprm). 3,4-dibromopyrrole-2,5-dione also increased the intracellular accumulation of hoechst 33342 in wild-type but not in transporter-deficient strains and prevented h33342 efflux (ic50 = 0.79 μg/ml or 3 μm), a hallmark of efflux pump inhibitor (epi) functionality. a metabolomic survey of 36 pseudoalteromonas isolates mapped the presence of primarily brominated metabolites only within the p. piscicida phylogenetic clade, where a majority of antibiotic activity was also observed, suggesting a link between halogenation and enhanced secondary metabolite biosynthetic potential. in sum, 3,4-dibromopyrrole-2,5-dione is a potent epi and deserves further attention as an adjuvant to enhance the effectiveness of existing antibiotics.
