Resume

• 2003

  Center for Sleep & Circadian Neurobiology

  Recruited

  supervised

  trained and managed workflow for two associates\nCompleted project on measuring the effects of different genetic backgrounds on sleep behavior in the fruit fly

  Drosophila melanogaster\nCreated RNA interference vectors based upon the Transgenic RNAi Project at Harvard Medical School protocols\nRan project to examine fruit fly mutants for abnormal sleep phenotypes. Directed 3 undergraduate students and 2 technicians in performance of genetic crosses and measuring sleep phenotype using video system\nDirected technician in measuring gene expression using quantitative PCR\nCollaborated with Associate Professor

  Drexel University

  on two studies involving the impact of chromatin modification enzymes on sleep using the fruit fly model\nServed as outside advisor on the thesis committee for two Drexel Ph.D. students. Mentored both students on design and execution of sleep experiments for their thesis research\nDirected development of sleep analysis software with post doctoral fellow

  for use with analysis of video and infra-red beam break activity data. Software developed at BioinfoRX

  Madison

  Wisconsin\nCompleted Phase I SBIR with Pinnacle Technology for development of an automated sleep deprivation device for use with fruit flies \nTrained and mentored visiting high school students

  Senior Research Investigator

  University of Pennsylvania School of Medicine

  University of Pennsylvania

• 1999

  University of Pennsylvania School of Medicine

  University of Pennsylvania

  Greater Philadelphia Area

  Prepare and present lectures on Genetics and Molecular Biology at an intermediate level and a two semester Introductory Biology course covering cell

  biological molecules

  Mendelian genetics

  evolutionary theory

  comparative physiology of cells and ecology. Write and grade midterms and final exams.\n Also academic advisor for post-baccalaureate students pursuing a pre-health certificate in the College of Liberal and Professional Studies.

  Lecturer A

  University of Pennsylvania

  Center for Sleep & Circadian Neurobiology

  Developed video based sleep monitoring system for use in the fruit fly

  in collaboration with Assistant Professor of Neurology and Teaching Assistant at Columbia University\n Published first paper proposing video behavioral recording to be used as the gold standard\nCompleted microarray study on messenger RNA changes during sleep/wake in fruit fly brain\n First study to examine brain specific RNA changes with sleep in the fly\n Supervised and trained research technician in RNA isolation and dissection of fly brains\nCompleted study on glycogen levels in the brain of the fruit fly during sleep/wake\nContributed to project using HPLC analysis to measure adenosine in specific areas of rat brain\nInterpreted research findings and summarized data for internal laboratory presentations.\nPresented research findings at international

  regional scientific conferences and peer reviewed journals

  Research Specialist

  University of Pennsylvania School of Medicine

  Philadelphia

  Prepare and present lectures on Genetics and Molecular Biology at an intermediate level.\nWrite and grade two midterm and final exams.

  Lecturer B

  University of Pennsylvania/ College of Liberal & Professional Studies

• 1984

  PhD

  Molecular Biology

  Temple University

• 1978

  Bachelor of Science (BS)

  Biology

  General

  Penn State University

• Cell Biology

  Immunohistochemistry

  Fluorescence Microscopy

  Product Development

  Molecular Biology

  PCR

  Proofreading

  Protein Expression

  Microsoft Office

  Immunoprecipitation

  Confocal Microscopy

  qPCR

  Animal Models

  Molecular Cloning

  Management

  Laboratory

  Immunofluorescence

  Western Blotting

  Flow Cytometry

  Behavior Analysis

  A video method to study Drosophila sleep

  Allan Pack

  David Raizen

  Video digital analysis is more accurate than single infra red beam break methods for fly sleep measurements and also permits the assessment of fly position and brief movements during sleep. In particular

  conclusions drawn from single beam infra red beam measurements regarding daytime sleep and sleep architecture should be made with caution.

  A video method to study Drosophila sleep

  The impact of age on the enzymatic activities of adenosine metabolic enzymes

  i.e.

  adenosine deaminase

  adenosine kinase

  cytosolic- and ecto-5′-nucleotidase have been assessed in the brain sleep/wake regulatory areas of young

  intermediate and old rats (2

  12 and 24 months

  respectively).

  Age-related changes in adenosine metabolic enzymes in sleep/wake regulatory areas of the brain

  Allan Pack

  Gary Churchill

  Mirek Mackiewicz

  Nirijini Naidoo

  David Raizen

  Keith Shockley

  Wendy Rizzo

  Using ANOVA we have identified 252 genes that were differentially expressed between sleep-deprived and control groups in the Drosophila brain. Using linear trends analysis

  we have separated the significant differentially expressed genes into nine temporal expression patterns relative to a common anchor point. The most common expression pattern is a decrease during extended wakefulness but no change during spontaneous sleep. Genes in this category were involved in protein production

  calcium homeostasis

  and membrane excitability. Multiple mechanisms

  therefore

  act to limit wakefulness. In addition

  by studying the effects of the mechanical stimulus used in our deprivation studies during the period when the animals are predominantly active

  we provide evidence for a previously unappreciated role for the Drosophila immune system in the brain response to stress.

  Multiple mechanisms limit the duration of wakefulness in Drosophila brain

  Allan Pack

  Nicholas Jackson

  One must control environmental conditions in a rigorously consistent manner to ensure that sleep data may be compared between experiments. Genetic background has a significant impact upon changes in sleep behavior and variance of behavior due to demographic factors and environmental interventions. This represents an opportunity to discover new genes that modify sleep/wake behavior.

  Genetic background has a major impact on differences in sleep resulting from environmental influences in Drosophila.

  Allan Pack

  David Raizen

  Nirijini Naidoo

  We review the evidence for the presence of sleep states in non-mammalian species including zebrafish (Danio rerio)

  fruitflies (Drosophila melanogaster) and roundworms (Caenorhabditis elegans). We describe conserved sleep-regulatory molecular pathways with a focus on cAMP and epidermal growth factor signaling; neurotransmitters with conserved effects on sleep and wake regulation

  including dopamine and GABA; and a conserved molecular response to sleep deprivation involving the chaperone protein BiP/GRP78.

  Conservation of sleep: insights from non-mammalian model systems

  Aging induced endoplasmic reticulum stress alters sleep and sleep homeostasis

  John