Resume

• 2008

  Doctor of Philosophy (Ph.D.)

  Clinical and Bioanalytical Chemistry

  Cleveland State University

  Fellow of the National Academy for Clinical Biochemistry (NACB)

  National Academy for Clinical Biochemistry

  Diplomate of the American Board of Clinical Chemistry (DABCC)

  American Board for Clinical Chemistry

• 2005

  Bachelor of Science (B.Sc.)

  Graduated with Honors

  Chemistry

  Chemistry Student Society

  Cultural Development Club

  American University of Beirut

• Bioanalytical Chemistry

  Clinical

  IRB

  Column Chromatography

  LC

  Chromatography

  Immunoassays

  Mass Spectrometry

  IR spectroscopy

  Sample Preparation

  Toxicology

  Chemistry

  LC-MS

  Biochemistry

  Clinical Research

  Bioanalysis

  Clinical Trials

  Analytical Chemistry

  HPLC

  NMR

  Amina Abbadi

  Joe El-Khoury

  Sihe Wang. Hemoglobin A1c. In Applications of LC-MS in Clinical Labs: Practical Guidelines. Edited by Shuijun Li and Sihe Wang. Shanghai Scientific & Technical Publishers. 2014.

  Sihe Wang

  Amina Abbadi

  Joe El-Khoury

  Sihe Wang. Hemoglobin A1c. In Applications of LC-MS in Clinical Labs: Practical Guidelines. Edited by Shuijun Li and Sihe Wang. Shanghai Scientific & Technical Publishers. 2014.

  Sihe Wang

  Clinical testing for vitamin D nutritional status has experienced tremendous growth in the past several years

  driven by research results linking various diseases with low serum 25-hydroxyvitamin D [25(OH)D] levels. Meanwhile

  interest in the pathophysiological mechanism elucidation and pharmaceutical applications requires measurement of vitamin D metabolites and analogues. Liquid chromatography-mass spectrometry (LC-MS) has been increasingly utilized in these applications. In this work

  our objective was to critically review the progress of LC-MS application in measuring vitamin D metabolites and analogues in biological fluids.The LC-MS methods included were selected from those searchable in PubMed up to January 2010.\nLC-MS has unique advantages in measuring various vitamin D metabolites and analogues due to its flexibility

  sensitivity

  and specificity. Despite some controversies over serum 25(OH)D tests

  LC-MS will be used for standardizing serum 25(OH)D assays using reference materials available from the National Institute of Standards and Technology.

  Progress of liquid chromatography-mass spectrometry in measurement of vitamin D metabolites and analogues.

  Sihe Wang

  Author(s):\n Kazmierczak

  Steven C

  editor\n Azzazy

  Hassan M E

  editor\n Lott

  John A (John Alfred)1936-Clinical enzymology\nTitle(s):\n Diagnostic enzymology / edited by Steven Kazmierczak

  Hassan M.E. Azzazy.\nUniform Title:\n Diagnostic enzymology (Kazmierczak) \nSeries:\n De Gruyter textbook\nEdition:\n 2nd fully revised and extended edition.\nCountry of Publication:\n Germany\nPublisher:\n Berlin : Walter de Gruyter GmbH & Co. KG

  [2013]\nLanguage:\n English\nISBN:\n 9783110207248 (alk. paper)\nLCCN:\n 2013045278\nMeSH:\n Clinical Enzyme Tests*\n Enzymes/analysis\nPublication Type(s): \n Case Reports\nNotes: \n Preceded by Clinical enzymology / John A. Lott

  Paul L. Wolf. New York City

  N.Y. : Field

  Rich

  and Associates

  c1986.\nContents:\n Angiotensin converting enzyme (ACE) / by Joe M. El-Khoury

  Edmunds Reineks and Sihe Wang -- Acetylcholinesterase and butyrylcholinesterase / by Peter L. Platteborze -- Aldolase / by Sihe Wang -- Alkaline phosphatase / by Amy E Schmidt -- Aspartate aminotransferase and alanine aminotransferase / by Joe M. El-Khoury and Sihe Wang -- Brain natriuretic peptide / by Robert H. Christenson and Hassan M.E. Azzazy -- Creatine kinase isoenzymes and isoforms / by Alan H.B. Wu -- Gamma-glutamyl transferase / by Sarah M. Brown -- Lactate dehydrogenase / by Olajumoke Oladipo and Dennis J. Dietzen -- Pancreatic lipase / by Wan-Ming Zhang

  Edmunds Reineks

  Joe M. El-Khoury and Sihe Wang.\nNLM ID: \n 101620098 [Book]

  Book Chapter: Angiotensin Converting Enzyme in Diagnostic Enzymology

  Sihe Wang

  Jessica Gabler

  Measurement of GFR for Children and an LC-MS/MS method for Nonradioactive Iothalamate.

  Development and validation of mass spec based assay in quantifying vitamin D

  without the interference of its C3-epimer.

  High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations

  18 minute peer-reviewed recorded presentation on \"Blood Gases\" as part of the \"Pearls of Laboratory Medicine\" series in Clinical Chemistry Trainee Council.

  Blood Gases

  Tobacco smoking is a major global health issue and represents the leading cause of preventable death in the developed countries. Nicotine is a major alkaloid found in tobacco products and its detection with its metabolites in human matrices is generally used for assessing tobacco consumption and second hand exposure. Several analytical techniques have been developed for the detection of nicotine and its metabolites

  and MS coupled with chromatography is considered the standard reference method because of its superior sensitivity and specificity. In this work

  we reviewed nicotine metabolism

  clinical MS and the latest (2009-2014) development of MS-based techniques for measurement of nicotine and metabolites in human matrices. Appropriate biomarker and matrix selection are also critically discussed.

  Recent advances in MS methods for nicotine and metabolite analysis in human matrices: clinical perspectives.

  Sihe Wang

  Raymond Jackson

  Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase and an established biomarker for endothelial function

  while symmetric dimethylarginine (SDMA)

  an emerging biomarker for renal function

  has been shown to outperform creatinine-based equations for estimated glomerular filtration rate. In order to study these analytes for clinical research

  a fast and simple method for measuring arginine (ARG)

  SDMA

  and ADMA in plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Plasma (50 μL) was mixed with 50 μL of internal standard of (13)C-arginine and d(7)-ADMA followed by protein precipitation with methanol containing 1% ammonium acetate (300 μL). After centrifugation

  the supernatant (100 μL) was mixed with 300 μL of acetonitrile with 1% formic acid

  and the mixture was injected onto a silica column monitored by a mass spectrometer. The analytical cycle time was 5.0 min. The method was linear from 5.7 to 489.7 μM for ARG

  0.06 to 5.15 μM for SDMA

  and from 0.34 to 5.65 μM for ADMA

  with an accuracy of 99.0-120.0%. Total coefficients of variation for all analytes ranged from 2.7% to 7.7% for three concentration levels. The effects of hemolysis

  lipemia

  uremia

  icterus

  specimen tube types

  storage at different temperature

  and freeze/thaw were thoroughly investigated. Reference ranges were established using 51 well-defined reference subjects (12 men and 39 women

  age 19-64 years): 53.1-129.7 μM for ARG

  0.32-0.65 μM for SDMA

  and 0.36-0.67 μM for ADMA. In conclusion

  the validated LC-MS/MS method described here offers a fast and reliable ARG

  SDMA

  and ADMA quantitation in plasma with minimum sample preparation.

  A simple and fast liquid chromatography-tandem mass spectrometry method for measurement of underivatized L-arginine

  symmetric dimethylarginine

  and asymmetric dimethylarginine and establishment of the reference ranges.

  Sihe Wang

  In this study we investigated the effect of hemolysis on plasma ammonia measurement using two different techniques (osmotic shock and shearing). The two techniques used to generate the hemolysis showed contradicting results. Further investigation revealed that the shearing technique was the more accurate method to use to generate hemoylsis for this study. Hemolysis has a lesser effect on the measurement of plasma ammonia than reported by the manufacturer.

  Is the effect of hemolysis on plasma ammonia measurement overrated?

  Sihe Wang

  Chao Yuan

  Does Paricalcitol (Zemplar®) interfere with 1

  25-dihydroxyvitamin D measurement by liquid chromatography-tandem mass spectrometry assays?

  Sihe Wang

  Jessica Gabler

  Do deuterium labeled internal standards correct for matrix effects in LC-MS/MS assays? A case study using plasma free metanephrine and normetanephrine.

  Sihe Wang

  Amina Abbadi

  Clinical Biochemistry

  Stability of serum and plasma osmolality in common clinical laboratory storage conditions.

  Sihe Wang

  Author(s):\nKazmierczak

  Steven C

  editor\nAzzazy

  Hassan M E

  editor\nLott

  John A (John Alfred)1936-Clinical enzymology\nTitle(s):\nDiagnostic enzymology / edited by Steven Kazmierczak

  Hassan M.E. Azzazy.\nUniform Title:\nDiagnostic enzymology (Kazmierczak)\nSeries:\nDe Gruyter textbook\nEdition:\n2nd fully revised and extended edition.\nCountry of Publication:\nGermany\nPublisher:\nBerlin : Walter de Gruyter GmbH & Co. KG

  [2013]\nLanguage:\nEnglish\nISBN:\n9783110207248 (alk. paper)\nLCCN:\n2013045278\nMeSH:\nClinical Enzyme Tests*\nEnzymes/analysis\nPublication Type(s):\nCase Reports\nNotes:\nPreceded by Clinical enzymology / John A. Lott

  Paul L. Wolf. New York City

  N.Y. : Field

  Rich

  and Associates

  c1986.\nContents:\nAngiotensin converting enzyme (ACE) / by Joe M. El-Khoury

  Edmunds Reineks and Sihe Wang -- Acetylcholinesterase and butyrylcholinesterase / by Peter L. Platteborze -- Aldolase / by Sihe Wang -- Alkaline phosphatase / by Amy E Schmidt -- Aspartate aminotransferase and alanine aminotransferase / by Joe M. El-Khoury and Sihe Wang -- Brain natriuretic peptide / by Robert H. Christenson and Hassan M.E. Azzazy -- Creatine kinase isoenzymes and isoforms / by Alan H.B. Wu -- Gamma-glutamyl transferase / by Sarah M. Brown -- Lactate dehydrogenase / by Olajumoke Oladipo and Dennis J. Dietzen -- Pancreatic lipase / by Wan-Ming Zhang

  Edmunds Reineks

  Joe M. El-Khoury and Sihe Wang.\nNLM ID:\n101620098 [Book]

  Book Chapter: Aspartate Aminotransferase and Alanine Aminotransferase in Diagnostic Enzymology

  Highly sensitive and selective measurement of underivatized methylmalonic acid in serum and plasma by liquid chromatography-tandem mass spectrometry.

  Sihe Wang

  Regina Spatholt

  Jessica Gabler

  Chao Yuan

  Methylmalonic acid (MMA) is a functional biomarker of vitamin B12 deficiency. Measurement of plasma MMA is challenging due to its small molecular weight and hydrophilic nature. Several liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods have been developed for measuring plasma MMA. However

  these methods involve lengthy sample preparation

  long chromatographic run time

  inadequate sensitivity

  or interference from succinic acid (SA). Here we report a novel LC-MS/MS method for quantitation of underivatized MMA in serum or heparinized plasma with high sensitivity and selectivity. Sample preparation involved only strong anion exchange solid phase extraction. The extract was purified by online turbulent flow and analyzed on an Organic Acids column. MS/MS analysis was performed in negative electrospray mode

  and the analytical time was 6 min. The use of ion ratio confirmation in combination with chromatographic resolution from SA greatly enhanced the selectivity. No interference was observed. This method was linear from 26.2 to 26

  010.0 nM with an accuracy of 98-111 %. Total coefficient of variation was less than 4.6 % for three concentration levels tested. Comparison with a reference laboratory LC-MS/MS method using leftover patient serum specimens (n = 48) showed a mean bias of -2.3 nM (-0.61 %) with a Deming regression slope of 1.016

  intercept of -6.6 nM

  standard error of estimate of 25.3 nM

  and a correlation coefficient of 0.9945. In conclusion

  this LC-MS/MS method offers highly sensitive and selective quantitation of MMA in serum and plasma with simple sample preparation.

  Highly sensitive and selective measurement of underivatized methylmalonic acid in serum and plasma by liquid chromatography-tandem mass spectrometry.

  Sihe Wang

  In this study we investigated the stability of 1

  25-dihydroxyvitamin D2 and 1

  25-dihydroxyvitamin D3 in human serum at physiological concentrations and under different storage conditions (room temperature and refrigerated). A liquid chromatography-tandem mass spectrometry method was used to measure the analytes. Both forms of 1

  25-dihydroxyvitamin D were stable for at least 2 weeks under all conditions tested.

  Stability of 1

  25-dihydroxyvitamin D(2) and 1

  25-dihydroxyvitamin D(3) in human serum.

  Joe

  Yale New Haven Health

  Cleveland State University

  Yale University School of Medicine

  Cleveland State University

  Cleveland Clinic

  American University of Beirut

  cleveland/akron

  ohio area

  Clinical Chemistry Post-doctoral Fellowship

  Cleveland Clinic

  Cleveland

  OH (July 2012-June 2014)\n•\tActing Director of Automated Chemistry and Special Chemistry\no\tReview and sign-out cases (ex: Hemoglobin A1c and Plasma metanephrine testing)\no\tOther duties as assigned by the medical directors\n•\tMain Research Focus: \no\tMethod development and validation by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the measurement of clinically relevant small molecules in biological fluids\no\tClinical correlation studies for emerging cardiac and kidney biomarkers\no\tVitamin D and chronic kidney disease\no\tClinical trials study coordinator: \no\tLarge multi-center study evaluating a LC-MS/MS assay for the measurement of immunosuppressant drugs by Thermo Fisher Scientific.\no\tLarge multi-center study evaluating an automated immunoassay for the measurement of immunosuppressant drugs by Roche Diagnostics.\no\tNon-targeted biomarker discovery in collaboration with Berg Diagnostics.\no\tRecruitment of healthy volunteers for a large reference range study.\n•\tAcademic Duties:\no\tLecturer at the Cleveland Clinic Clinical Pathology Didactic Conference.\no\tLecturer at the Cleveland Clinic School of Medical Technology.\no\tOrganizer and lecturer at “Test of the Week” and “Clinical Chemistry Journal Club” education series for residents

  fellows and medical technologists.\n•\tClinical Rotations: \no\tRotated in: Quality Assurance and Method Development

  STAT Chemistry and Point of Care Testing

  Automated Chemistry

  Immunoassay lab

  Molecular Diagnostics

  Toxicology and Therapeutic Drug Monitoring

  Information Systems

  Pediatric Clinical Chemistry and Inborn Errors of Metabolism

  Lipids

  Inherited Diseases

  Safety

  Regulations

  Compliance

  Management and Billing

  Microbiology

  and Blood Bank.\n•\tSupervisory Duties: \no\tManage research projects of incoming Clinical Chemistry PhD students from Cleveland State University.\no\tAssist and manage residents rotating in chemistry with their on-call duties.

  Clinical Chemistry Post-Doctoral Fellow

  Cleveland Clinic

  Cleveland

  OH

  • Planned and taught undergraduate general chemistry laboratory I and II.\n• Tutored biochemistry

  organic chemistry and general chemistry to undergraduate students.

  Teaching Assistant

  Cleveland State University

  Cleveland

  Ohio

  PhD Dissertation

  Cleveland State University

  Cleveland

  OH (2008-2012).\n• Designed and conducted a clinical trial to investigate the effects of different vitamin D formulations and treatment strategies on chronic kidney disease patients. The written proposal was approved by Cleveland Clinic’s Institutional Review Board (IRB) and awarded a grant by its Research Protocol Committee (RPC).\n• Developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to measure iothalamate in serum and urine.\n• Validated a LC-MS/MS method for the measurement of methylmalonic acid in serum and plasma.\n• Developed and validated a LC-MS/MS method for the measurement of arginine and dimethylated derivatives in plasma.\n• Investigated pre-analytical factors impacting arginine and dimethylated derivatives and established their reference ranges.\n• Investigated clinical correlations between arginine and dimethylated derivatives with various cardiovascular and kidney biomarkers.\n• Investigated the stability of 1

  25-dihydroxyvitamin D in serum.\n• Co-author of the LC-MS/MS method validation protocol written for Cleveland Clinic’s Pathology and Laboratory Medicine Institute.\n• Received LC-MS/MS training on Agilent QQQ 6460 (MassHunter software)

  Thermo TSQ Quantum Access (Xcalibur and Aria software)

  and Applied Biosystems 5500 QTrap (Analyst software).

  PhD Candidate in Clinical and Bioanalytical Chemistry

  Cleveland Clinic

  New Haven

  Connecticut

  United States

  Associate Professor of Laboratory Medicine

  Yale University School of Medicine

  Yale New Haven Health

  Assistant Professor of Laboratory Medicine

  Yale University School of Medicine

  Cleveland State University

  cleveland/akron

  ohio area

  Lecturer: Undergraduate level college chemistry (CHM 251) and general chemistry (CHM 261).

  Adjunct Faculty

  Yale New Haven Health

  Yale New Haven Health

  New Haven

  Connecticut

  Co-Director of Clinical Chemistry

  Beirut

  Lebanon

  • Designed and performed bio-guided fractionation of anti-inflammatory compound from a Lebanese endemic plant.\n• Purified bioactive molecules using liquid-liquid extraction

  solid phase extraction and column chromatography.\n• Identified the molecular structure of unknown compounds with biological activities using 2D-NMR

  1H-NMR

  13C-NMR

  GC-MS

  and IR.\n• Investigated the structure-activity relationship of sesquiterpene lactones to anti-tumor activity.\n• Trained incoming research assistants and PhD candidates on the use of spectroscopic instruments.

  Research Assistant

  American University of Beirut

  The purpose of the DMG is to advocate the role of AACC divisions in the educational mission of the association

  facilitate the operations of divisions in the association and counsel and advise divisions on association policies and procedures. In addition

  the DMG should facilitate communication between division leadership and division members (via newsletter

  blog

  listserv or other means).

  Member

  Division Management Group (DMG) of the American Association for Clinical Chemistry (AACC)

  The Pediatric and Maternal-Fetal Division provides a forum for the dissemination of information about analytes

  methods of analysis

  and reference ranges for pediatric and maternal-fetal patients. The Division encourages research in specialized areas of pediatric

  maternal and fetal clinical chemistry.\n\nLink to division:\nhttp://www.aacc.org/members/divisions/pediatrics/pages/default.aspx#

  Board Member

  Pediatric and Maternal-Fetal Division

  Multiple Positions held:\nEducation Coordinator: January 2014-present\nStudent Member January 2012-August 2013\n\nDescription:\nThe Northeast Ohio Local Section serves AACC members and clinical chemists in the region.\n\nLink to local section:\nhttp://www.aacc.org/members/loc_sections/northeastohio/pages/default.aspx#

  Education Coordinator

  Northeast Ohio-American Association for Clinical Chemistry

  SYCL360 is a podcast designed for SYCL members. We are planning on releasing SYCL360 quarterly to keep you up-to-date on pertinent information for SYCL members

  highlight recent SYCL activities

  as well as to promote inter-SYCL member communication.\n\nLink to podcasts:\nhttp://www.aacc.org/members/sycl/sycl360/pages/default.aspx#

  Chair

  SYCL 360 Online Podcast Subcommittee

  Board Director

  Clinical Laboratory Management Association

  SYCL stands for the Society for Young Clinical Laboratorians

  a special program to serve the needs of younger AACC members.\n\nLink to SYCL:\nhttp://www.aacc.org/members/sycl/pages/default.aspx#

  Member

  Society for Young Clinical Laboratorians (SYCL)

  Member

  Academy of Clinical Laboratory Physicians and Scientists

  AACC is a leading professional society dedicated to improving healthcare by advancing knowledge

  expertise

  and innovation in laboratory medicine.\n\nAACC members are clinical laboratory professionals

  physicians

  research scientists and others involved in developing tests and directing laboratory operations. They work in all areas of laboratory medicine—in clinical laboratories

  classrooms

  diagnostic companies

  and at the bedside. \n\nLink to AACC:\nwww.aacc.org

  Member

  American Association for Clinical Chemistry (AACC)

  The role of the Ohio-CLC committee is to organize the annual Ohio-CLC conference. This year

  the conference will be taking place in Columbus

  OH from May 8th till May 10th. This conference is organized by the Northeast Ohio-American Association for Clinical Chemistry (NEO-AACC)

  American Society for Clinical Laboratory Science-Ohio (ASCLS-OHIO)

  and Clinical Laboratory Management Association (CLMA) and attracts around 200 attendees every year from all over Ohio

  including pathologists

  laboratory directors

  medical technologists

  lab managers and students from various disciplines.\n\nLink to program:\nhttp://www.cvent.com/events/collaborative-laboratory-conference-2013/event-summary-d63a4dbb3bd4473c9c42be430134af76.aspx

  Chair

  Program Subcommittee

  Ohio-Collaborative Laboratory Conference (CLC) Committee

  English

  Arabic

  French

  Graduate Student Fellowship Award for an Outstanding Manuscript in Applied Science and Technology

  Lerner Research Institute

  Cleveland Clinic

  Cleveland

  OH

  2013 Clinical Chemist Recognition Award

  American Association for Clinical Chemistry

  American Association for Clinical Chemistry's Past-Presidents' Scholarship

  Link to award description and announcement:\n\nhttp://www.aacc.org/about/vsf/awards_grants/Pages/Past-Presidents%20Scholarships.aspx#

  American Association for Clinical Chemistry

  Trainee Travel Award

  Mass Spectrometry Applications to the Clinical Lab (MSACL)

  San Diego

  CA

  SYCL Domestic Travel Grant

  Society for Young Clinical Laboratorians (SYCL)

  Young Investigator Award

  Mass Spectrometry Applications to the Clinical Lab (MSACL)

  San Diego

  CA

  Teaching Award

  Presented in Recognition of Outstanding Teaching in the Laboratory Medicine Residency Program

  Yale University School of Medicine

  Student Travel Grant Award

  American Association for Clinical Chemistry

  2015 Young Investigator Award

  Mass Spectrometry Applications to the Clinical Lab (MSACL)

  AACC Outstanding Speaker Award

  American Association for Clinical Chemistry

  2012 Clinical Chemist Recognition Award

  American Association for Clinical Chemistry

  First Place

  F. Merlin Bumpus Ph.D. Junior Investigator Award in Clinical Research

  F. Merlin Bumpus Junior Investigator Awards are presented at the annual Cleveland Clinic Research Day to recognize outstanding contributions submitted by junior scientists in training (Postdoctoral Fellows and Graduate Students) for presentation at the event. The abstracts and oral presentations of the semifinalists are judged by the Research Day abstract review committee.\n\nLink to description:\nhttp://www.lerner.ccf.org/education/grad/

  Cleveland Clinic