Resume

• 1999

  Texas A&M University

  Galveston

  Texas

  Principal Investigator

  Texas A&M University

  Texas A&M University

  Texas A&M University

  Galveston

  TX

  Assistant Professor

  Galveston

  Texas

  Interim Department Head - Department of Marine Biology

  Texas A&M University

  Galveston

  Texas

  Associate Professor

  Texas A&M University at Galveston

• 1993

  University of South Carolina

  Texas A&M University at Galveston

  University of South Carolina

• 1988

  Doctor of Philosophy (Ph.D.)

  Ichthyology & Genetics

  University of Toronto

• 1985

  Master's degree

  Numerical Analysis

  University of Toronto

• 1978

  Licenciatura

  Zoology/Biology

  Universidad Autonoma Metropolitana - Iztapalapa

• Scientific Writing

  Oceanography

  Molecular Biology

  Environmental Education

  Marine Conservation Biology

  Maritime

  Field Work

  Genetics

  Aquatic Ecology

  Molecular Systematics

  Ecology

  Biology

  Marine Biogeography

  Population Genetics

  Molecular Genetics

  Environmental Science

  Science

  Experimental Design

  Marine Ecology

  Marine Biology

  Methodological streamlining of SNP discovery and genotyping via high-resolution melting analysis (HRMA) in non-model species

  Ching-Ping Lu

  Brad L. Smith

  Here we provide a rapid and low-cost workflow utilizing high-resolution melting analysis (HRMA) for nuclear marker development and genotyping of 774 Atlantic and Mediterranean swordfish (Xiphias gladius) that is amendable to other species. Preliminary HRMA screening of amplicons (>290 bp) for 10 nuclear loci revealed the presence of nucleotide polymorphisms

  however

  length and variability precluded diagnostic genotyping. Two variants of HRMA were therefore utilized to provide diagnostic genotyping assays. Short-amplicon HRMA (SA-HRMA)

  in which primers flank closely a SNP of interest

  was identified as a low cost

  rapid

  closed-tube diagnostic genotyping assay that could distinguish between homozygous genotypes by ΔTm

  and heterozygous genotypes by heteroduplex melting curve profiles. When the patterns of sequence variation were not suitable for SA-HRMA

  unlabeled probe (UP)-HRMA was utilized. UPHRMA has the advantage of being capable of genotyping multiple linked SNPs in a single closed-tube assay without Bayesian haplotype reconstruction

  and can identify new SNPs while genotyping populations.

  Methodological streamlining of SNP discovery and genotyping via high-resolution melting analysis (HRMA) in non-model species

  Rosa Flos

  Pedro Pablo Ambrosio

  Jordi Viñas

  Loss of genetic variability in a hatchery strain of Senegalese sole (Solea senegalensis) revealed by sequence data of the mitochondrial DNA control region and microsatellite markers

  Ditty

  James

  Species discrimination of postlarvae and early juvenile brown shrimp (Farfantepenaeus aztecus) and pink shrimp (Farfantepenaeus duorarum) (Decapoda:Penaedidae): Coupling molecular genetics and morphology to identify early life stages.

  Aid in the collection and banding of nestling juvenile Caribbean Flamingo to improve wildlife management and conservation of the populations of this species.

  Development of Loop Mediated Isothermal DNA Amplification assays for tuna identification

  Alvarado Bremer