Resume

• 1999

  O'Connell

  St. Andrew's School of Delaware

  SomaLogic

  Inc

  NeXstar Pharmaceuticals

  NeXstar Pharmaceuticals

  SomaLogic

  Inc

  Boulder

  Colorado

  Visiting Scientist

  •Teacher of Biology

  Advanced Biology

  Chemistry

  Environmental Science

  Cognitive Psychology

  Contemporary American Law

  and several other courses.\n•Coach and attorney advisor of the mock trial team.\n•Head coach of the boy's cross country team.

  St. Andrew's School of Delaware

• 1996

  Doctor of Law (J.D.)

  Law

  Licensed to practice law in Colorado since 2001.

  University of Colorado

• 1990

  M.A.

  Molecular Biology

  University of Colorado

• 1986

  BS

  Biology

  Haverford College

  Honors

• Teaching

  Lifesciences

  Assay Development

  Biology

  Chemistry

  Law

  Research

  Microsoft Office

  Biochemistry

  Biotechnology

  Science

  Public Speaking

  Nonprofits

  Cell Culture

  Data Analysis

  Protein Chemistry

  PCR

  Molecular Biology

  Editing

  Drug Discovery

  Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets.

  Lance J Stewart

  Larry Gold

  Nebojsa Janjic

  Thale C Jarvis

  Sheela M Waugh

  Jeffrey D Carter

  John C Rohloff

  Chi Zhang

  Amy D Gelinas

  Douglas R Davies

  My principle contribution to this manuscript was to develop and perform cell-culture-based PDGF activity assays. I then tested DNA aptamers for their ability to inhibit PDGF activity.

  Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets.

  Mel Santer

  This report describes the functional effects of a single base change in a highly conserved loop of Escherichia coli ribosomal RNA.

  Functional effects of a G to U base change at position 530 in a highly conserved loop of Escherichia coli 16S RNA.

  Parma DH

  Ringquist S

  Weigand L

  Chang YF

  Watson SR

  My principle contribution to this manuscript was to perform the in vitro selection-amplification that generated the anti-L-selectin aptamers that were used in subsequent pharmacokinetic and activity assays.

  Anti-L-selectin aptamers: binding characteristics

  pharmacokinetic parameters

  and activity against an intravascular target in vivo

  Y F Chang

  P Bridonneau

  D Parma

  A V Buvoli

  My principle contribution to this manuscript was to develop and perform a novel vitro selection-amplification (SELEX) protocol. This method was successful in producing aptamers with calcium-dependent affinity for their target.

  Site-directed selection of oligonucleotide antagonists by competitive elution

  Philippe Bridonneau Ying-Fon Chang † Stanley C. Gill David W. Snyder Lea Johnson Theodore Goodson Jr

  This report describes the isolation of an inhibitor of human nonpancreatic secretory phospholipase A2.

  High-Affinity Aptamers Selectively Inhibit Human Nonpancreatic Secretory Phospholipase A2 (hnps-PLA2)

  Mel Santer

  In an effort to understand ribosome structure and function we investigated the multiple effects of introducing a base changes at position 792 of Escherichia coli 16S rRNA.

  Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes

  A Varki

  D Parma

  N Varki

  Y F Chang

  T Fitzwater

  H L Han

  B Hicke

  S Jennings

  A Koenig

  I am the first author for this publication

  which reports the development of high-affinity oligonucleotide aptamers specific for L-selectin. These antagonists show little binding to E- or P-selectin. Moreover

  they show calcium-dependent binding to native L-selectin on peripheral blood lymphocytes and block L-selectin-dependent interactions with the natural ligands on high endothelial venules. with high endothelial venules

  Calcium-dependent oligonucleotide antagonists specific for L-selectin