Texas A&M University

Assistant Professor

Corey John worked at Texas A&M University as a Assistant Professor

Massachusetts Institute of Technology (MIT)

Postdoctoral Associate

My post-doctoral work involved developing technology aimed to benefit the developing world. I was involved in the development of a single injection vaccine against T. solium, capable of providing its own booster at a later point in time, removing the need to meet the patient more than once. We tested this technology in a murine model.

The Janssen Pharmaceutical Companies of Johnson & Johnson

Senior Scientist

Corey John worked at The Janssen Pharmaceutical Companies of Johnson & Johnson as a Senior Scientist

Johns Hopkins Medicine

Postdoctoral Associate

My post-doctoral training at Hopkins was an extension of doctoral research.

Johns Hopkins Medicine

National Science Foundation Graduate Research Fellow

This work details how polymer structure of poly(β-amino ester)s (PBAE) affects polymer-DNA binding and how binding affects transfection levels, viability, and nanoparticle physical properties (zeta potential and diameter). We also investigated the comparative binding strengths of branched and linear polyethyleneimine, poly(L-lysine) and PBAEs with plasmid DNA and found PBAEs have the weakest binding. This work also details new bioassays including a more high throughput method for assessing cellular and nuclear uptake rates using flow cytometry. This method may be used for elucidating structure-function relationships in various cell types. An auto-fitting, first order mass-action kinetic model was developed in MatLab to quantify the intracellular delivery rate constants for comparing delivery bottlenecks of various polymer structures in various cell lines. This model was used to assess rate differences between polymers which do not transfect well, tansfect mediocre, and transfect well in primary human glioblastoma in vitro. The model recapitulated the experimental data with good agreement without needing to extrapolate data from literature. Principal component analysis is a method to look at large data sets with unknown variable correlations and to quantify how each variable is correlated with another, as well as which and to what degree each variable may drive another. Principal component analysis was utilized to look at 27 physico-chemical properties and cell gene delivery outcomes (i.e., uptake, transfection levels, and viability). We found that certain key parameters, such as hydrophobicity, drove uptake and transfection. The theranostic-enabling technology was capable of co-delivering DNA and siRNA as well as delivering two layers of DNA with two different expression time profiles. Co-delivering DNA and siRNA could allow for the knockdown of a dysfunctional aberrant protein while replacing it with a functional protein.

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

The Annals of Biomedical Engineering

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

The Annals of Biomedical Engineering

Refilling mechanism to stabilize a free-floating intraocular capsule drug ring (CDR)

2010 AIChE Annual meeting poster section: Engineering Fundamentals in Life Science

In 2009, 15.1 million cataracts were extracted and replaced with intraocular lenses (IOL). Because IOLs are smaller in diameter than natural lenses, there is real estate in the periphery of the IOL unused. The Capsule Drug Ring (CDR) is an implantable device that stores and releases drug inside the capsular bag in this unused periphery. The objective of the refilling mechanism is to stabilize a free-floating body to allow penetration through the refilling ports. Two ports at each ends of the CDR allow the reservoir to be refilled with bevacizumab (Avastin) every six months to one year. Avastin is an antivascular endothelial growth factor which inhibits blood vessel proliferation. The maximum width of the refilling mechanism is about 23 gauge. The 23 gauge refilling device will constitute an inner 30 gauge needle which will penetrate the ports, injecting Avastin into the CDR reservoir. Lasso loop is applied to grab and fix CDR while refilling. There are several structures on the CDR such as lasso guiding loop and protection wall to allow lasso grabbing mechanism. We developed several shapes of loop to ease the refilling process since it will be operated by normal ophthalmologist.

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

The Annals of Biomedical Engineering

Refilling mechanism to stabilize a free-floating intraocular capsule drug ring (CDR)

2010 AIChE Annual meeting poster section: Engineering Fundamentals in Life Science

In 2009, 15.1 million cataracts were extracted and replaced with intraocular lenses (IOL). Because IOLs are smaller in diameter than natural lenses, there is real estate in the periphery of the IOL unused. The Capsule Drug Ring (CDR) is an implantable device that stores and releases drug inside the capsular bag in this unused periphery. The objective of the refilling mechanism is to stabilize a free-floating body to allow penetration through the refilling ports. Two ports at each ends of the CDR allow the reservoir to be refilled with bevacizumab (Avastin) every six months to one year. Avastin is an antivascular endothelial growth factor which inhibits blood vessel proliferation. The maximum width of the refilling mechanism is about 23 gauge. The 23 gauge refilling device will constitute an inner 30 gauge needle which will penetrate the ports, injecting Avastin into the CDR reservoir. Lasso loop is applied to grab and fix CDR while refilling. There are several structures on the CDR such as lasso guiding loop and protection wall to allow lasso grabbing mechanism. We developed several shapes of loop to ease the refilling process since it will be operated by normal ophthalmologist.

Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles

The Journal of Controlled Release

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

The Annals of Biomedical Engineering

Refilling mechanism to stabilize a free-floating intraocular capsule drug ring (CDR)

2010 AIChE Annual meeting poster section: Engineering Fundamentals in Life Science

In 2009, 15.1 million cataracts were extracted and replaced with intraocular lenses (IOL). Because IOLs are smaller in diameter than natural lenses, there is real estate in the periphery of the IOL unused. The Capsule Drug Ring (CDR) is an implantable device that stores and releases drug inside the capsular bag in this unused periphery. The objective of the refilling mechanism is to stabilize a free-floating body to allow penetration through the refilling ports. Two ports at each ends of the CDR allow the reservoir to be refilled with bevacizumab (Avastin) every six months to one year. Avastin is an antivascular endothelial growth factor which inhibits blood vessel proliferation. The maximum width of the refilling mechanism is about 23 gauge. The 23 gauge refilling device will constitute an inner 30 gauge needle which will penetrate the ports, injecting Avastin into the CDR reservoir. Lasso loop is applied to grab and fix CDR while refilling. There are several structures on the CDR such as lasso guiding loop and protection wall to allow lasso grabbing mechanism. We developed several shapes of loop to ease the refilling process since it will be operated by normal ophthalmologist.

Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles

The Journal of Controlled Release

A novel non-invasive method to assess aortic valve opening in HeartMate II patients using a modified Karhunen-Loève Transformation

Journal of Heart and Lung Transplantation

Gene delivery polymer structure-function relationships elucidated via principal component analysis

Chemical Communications

Independent vs cooperative binding in polyethyleneimine-DNA and poly(L-lysine)-DNA polyplexes

The Journal of Physical Chemistry Part B

Highlights from the latest articles in nanomedicine

The Journal of Nanomedicine

Nano-Gold/Degradable Polymer Hybrid Nanoparticles for Co-Delivery of DNA and siRNA

Acta Biomaterialia

Advances in Polymeric and Inorganic Vectors for Non-viral Nucleic Acid Delivery

Therapeutic Delivery

Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

The Annals of Biomedical Engineering

Refilling mechanism to stabilize a free-floating intraocular capsule drug ring (CDR)

2010 AIChE Annual meeting poster section: Engineering Fundamentals in Life Science

In 2009, 15.1 million cataracts were extracted and replaced with intraocular lenses (IOL). Because IOLs are smaller in diameter than natural lenses, there is real estate in the periphery of the IOL unused. The Capsule Drug Ring (CDR) is an implantable device that stores and releases drug inside the capsular bag in this unused periphery. The objective of the refilling mechanism is to stabilize a free-floating body to allow penetration through the refilling ports. Two ports at each ends of the CDR allow the reservoir to be refilled with bevacizumab (Avastin) every six months to one year. Avastin is an antivascular endothelial growth factor which inhibits blood vessel proliferation. The maximum width of the refilling mechanism is about 23 gauge. The 23 gauge refilling device will constitute an inner 30 gauge needle which will penetrate the ports, injecting Avastin into the CDR reservoir. Lasso loop is applied to grab and fix CDR while refilling. There are several structures on the CDR such as lasso guiding loop and protection wall to allow lasso grabbing mechanism. We developed several shapes of loop to ease the refilling process since it will be operated by normal ophthalmologist.

Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles

The Journal of Controlled Release

A novel non-invasive method to assess aortic valve opening in HeartMate II patients using a modified Karhunen-Loève Transformation

Journal of Heart and Lung Transplantation

Hypoxia-Inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis.

Proceedings of the National Academy of Sciences