Resume

• 1999

  English

  Korean

  PhD

  Molecular Biology

  University of Illinois at Chicago

• 1997

  MS

  Biology

  Illinois Institute of Technology

  Biotechnology

  Molecular Biology

• Purification of nucleic acid from mammalian cells and tissues

  Small RNA isolation

  Cell Biology

  Translational Research

  FACS Analysis

  Mammalian cell culture

  Quantitative real-time PCR-based RNA array

  Polyoma

  Retro and Lenti virus cloning and production

  Virus

  Immunofluorescence analysis of tissues

  Protein Purification

  Cancer Research

  Cancer

  Animal Models

  Detection of apoptotic cells with luciferase gene reporter and TUNEL assays

  in vitro/vivo ubiquitination assays

  Transformations

  Transfection and molecular cloning

  Analysis of Bisulfite converted DNAs with biotin pulldowns and pyrosequencing

  Cell Culture

  Chromatin immunoprecipitation

  Transcriptional and Post-translational Regulation of the Quiescence Factor and Putative Tumor Suppressor p150Sal2.

  Sung and Dahl contributed equally to this work

  Benjamin TL

  Rodig S

  Yim H

  Dahl J

  In both growing fibroblasts and established ovarian surface epithelial cells

  p150Sal2 undergoes polyubiquitination and proteosomal degradation. A CUL4/DDB1 E3 ligase containing RBBP7 as the p150Sal2 receptor has been identified as mediating the destruction of p150Sal2 as cells transition from a quiescent to an actively growing state.

  Transcriptional and Post-translational Regulation of the Quiescence Factor and Putative Tumor Suppressor p150Sal2.

  Polyoma Virus-Induced Osteosarcomas in Inbred Strains of Mice: Host Determinants of Metastasis.

  Velupillai and Sung contributed equally to this work

  Benjamin TL

  Bronson R

  Carroll J

  Dahl J

  Tian Y

  Velupillai P

  Comparing osteosarcoma cell lines from two different mouse starins

  we have identified a molecular pathway that underlies invasive behavior in vitro and correlates with metastasis in vivo.

  Polyoma Virus-Induced Osteosarcomas in Inbred Strains of Mice: Host Determinants of Metastasis.

  Sullivan and Sung contributed equally to this work

  Ganem D

  Benjamin TL

  Lukacher AE

  Grundhoff A

  Pack CD

  Sullivan CS

  We showed that PyV encodes a pre-miRNA during infection that is processed into two stable miRNAs

  and explored the functions of these miRNAs both in culture and experimental inoculation of mice.

  Murine Polyomavirus Encodes a MicroRNA That Cleaves Early RNA Transcripts But is Not Essential For Experimental Infection.

  Chang

  Sung

  Texas A&M University-Kingsville

  University of Illinois at Chicago

  Harvard Medical School

  Texas A&M University-Kingsville

  Research Fellow

  -Characterized ubiquitin-mediated regulation of a tumor suppressor p150Sal2\n-Identified and characterized a set of microRNAs encoded by the oncogenic polyoma virus\n-Analyzed the mechanisms of bone tumor metastasis during the mouse polyoma virus infection\n-Determined that the levels of TAZ and its kinase Nek1 are critical for polycystic kidney disease (PKD)

  Harvard Medical School

  Graduate Researcher

  -Developed new pneumococcal mutagenesis methods

  which have since been applied to many transformable streptococcal strains\n-Identified new genes in response to the competence pheromone by DNA microarrays\n-Performed functional analysis of ComW

  one of the new competence factors

  University of Illinois at Chicago

  Instructor

  -Guided and supervised junior researchers and students to develop various research projects\n-Screen de-ubiquitinating enzymes (DUBs) that may preserve a tumor suppressor p150Sal2\nin normal human ovarian surface epithelial cells\n-Examine epigenetic silencing (promoter methylation) of a tumor suppressor p150Sal2\nin human ovarian carcinomas\n-Screen murine non-coding RNAs in response to oncogenic polyoma virus infection\nto identify novel biomolecular markers involved in oncogenesis and tumor progression\n-Investigate how polymorphisms in toll-like receptor 4 (Tlr4) lead to different cytokine responses

  Harvard Medical School

  Associate Professor

  Texas A&M University-Kingsville