Brooke Napier

 BrookeA. Napier

Brooke A. Napier

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Biography

Portland State University - Biology


Resume

  • 2014

    Post Doctoral Fellowship

    Stanford University

    Science and Engineering Course Design

    Preparation & Practice: Biotech Business & Finance

  • 2008

    Doctor of Philosophy (PhD)

    My dissertation work focused on mechanisms used by pathogenic Francisella species and Acinetobacter baumannii use to evade the host immune response to replicate and cause disease.

    Microbiology and Immunology

    Emory University

  • 2003

    Bachelor of Arts (BA)

    Biopsychology (Neuroscience)

    Agnes Scott College

  • Animal Models

    Research

    Biochemistry

    Science

    Antibiotic Resistance

    Laboratory

    RT-PCR

    Western Blotting

    Molecular & Cellular Biology

    Microbiology

    Electron Microscopy

    Molecular Biology

    PCR

    Cell Culture

    Innate Immunity

    Molecular Cloning

    Cell Biology

    Tissue Culture

    Fluorescence Microscopy

    Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

    Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

    A link between biotin and rapid phagosomal escape in Francisella

    Colistin Heteroresistance in Enterobacter cloacae Is Associated with Cross-Resistance to the Host Antimicrobial Lysozyme

    Rogier Louwen

    Increasing the integrity of the bacterial envelope is necessary to allow the successful survival of bacterial pathogens within the host and allow them to counteract damage caused by membrane-targeting antibiotics. We demonstrate that components of a clustered

    regularly interspaced

    short palindromic repeats–CRISPR associated (CRISPR-Cas) system

    a prokaryotic defense against viruses and foreign nucleic acid

    act to regulate the permeability of the bacterial envelope

    ultimately providing these cells with the capability to resist membrane damage caused by antibiotics. This regulation further allows bacteria to resist detection by multiple host receptors to promote virulence. Overall

    this study demonstrates the breadth of function of CRISPR-Cas systems in regulation

    antibiotic resistance

    innate immune evasion

    and virulence.

    A CRISPR-Cas system enhances envelope integrity mediating antibiotic resistance and inflammasome evasion

    A Francisella virulence factor catalyses an essential reaction of biotin synthesis.

    Association of IS1016 with the hia adhesin gene and biotypes V and I in invasive nontypeable Haemophilus influenzae.

    Subversion of host recognition and defense systems by Francisella spp.

    Imatinib mesylate (Gleevec) inhibits Abl1

    c-Kit

    and related protein tyrosine kinases (PTKs) and serves as a therapeutic for chronic myelogenous leukemia and gastrointestinal stromal tumors. Imatinib also has efficacy against various pathogens

    including pathogenic mycobacteria

    where it decreases bacterial load in mice

    albeit at doses below those used for treating cancer. We report that imatinib at such low doses unexpectedly induces differentiation of hematopoietic stem cells and progenitors in the bone marrow

    augments myelopoiesis but not lymphopoiesis

    and increases numbers of myeloid cells in blood and spleen. Whereas progenitor differentiation relies on partial inhibition of c-Kit by imatinib

    lineage commitment depends upon inhibition of other PTKs. Thus

    imatinib mimics “emergency hematopoiesis

    ” a physiological innate immune response to infection. Increasing neutrophil numbers by adoptive transfer sufficed to reduce mycobacterial load

    and imatinib reduced bacterial load of Franciscella spp.

    which do not utilize imatinib-sensitive PTKs for pathogenesis. Thus

    potentiation of the immune response by imatinib at low doses may facilitate clearance of diverse microbial pathogens.

    Low Doses of Imatinib Induce Myelopoiesis and Enhance Host Anti-microbial Immunity

    Faculty of 1000

    Clinical use of colistin induces cross-resistance to host antimicrobials in Acinetobacter baumannii

    Bina

    J.E.

    Jones

    C.L.

    Replication Screen Identifies a Novel Francisella Hydroperoxide Resistance Protein Involved in Virulence.

    NaxD is a deacetylase required for lipid A modification and Francisella pathogenesis

    Hosted speakers. Coordinated flight and taxi schedules for visiting speakers. Also assisted in acquiring hotel accommodations.

    Elizabeth Ohneck

    Anne Hotard

    Stanford University

    Science Writer

    Agnes Scott College

    Psychology Department

    Emory University

    Portland State University

    Portland

    Oregon Area

    Biology Department

    Assistant Professor

    Portland State University

    Emory University

    Emory University

    Ph.D. Student - David Weiss Laboratory

    Agnes Scott College

    We worked on a project that assessed basal and post-stress corticosterone (CORT) levels

    an indicator of stress and inverse indicator of memory

    in adolescent rats. We investigated how maternal separation affected the relationship between CORT levels and rat social dominance.

    Research Assistant - Barbara Blatchley Laboratory

    Agnes Scott College

    Psychology Department

    Atlanta

    GA and Greenbank

    WV

    SmallerQuestions.org

    Science Writer

    Atlanta

    GA

    Lab Technician - Monica Farley Laboratory

    Emory University School of Medicine and Atlanta Veteran’s Hospital

    Stanford

    CA

    Post Doctoral Fellow - Denise Monack Laboratory

    Stanford University

    American Association of Immunologists

    American Association for the Advancement of Science

    English

    Katherine McCormick Advanced Postdoctoral Fellowship

    The Katharine McCormick Committee

    Stanford University

    NSF Graduate Fellowship Honorable Mention

    National Science Foundation

    The ITI Young Investigator Award

    Stanford Institute for Immunity

    Transplantation

    and Infection

    Young Investigator Novel Protocol Award

    Nature Protocols (Nature Publishing Group)

    Ruth I Kirschstein National Research Services Award (NRSA) Individual Postdoctoral Fellowship (F32)

    NIAID - National Institute of Health

    Keystone Global Health Travel Award

    Cell Death Signaling in Cancer and the Immune System Keystone Symposium Travel Award

    Keystone and São Paulo Research Foundation